![]() ![]() Probing/detection-incubation with antigen-specific antibodies that affinity-bind to the antigens.Plate blocking-addition of irrelevant protein or other molecule to cover all unsaturated surface-binding sites of the microplate wells.Coating/capture-direct or indirect immobilization of antigens to the surface of polystyrene microplate wells.This ability to use high-affinity antibodies and wash away non-specific bound materials makes ELISA a powerful tool for measuring specific analytes within a crude preparation.Īlthough many variants of ELISA have been developed and used in different situations, they all depend on the same basic elements: Having the reactants of the ELISA immobilized to the microplate surface makes it easy to separate bound from non-bound material during the assay. It is this binding and immobilization of reagents that makes ELISAs easy to design and perform. ELISAs are typically performed in 96-well or 384-well polystyrene plates, which passively bind antibodies and proteins. ![]() Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. You can also visit our ELISA builder tool, answer a series of questions, and be presented with recommendations on which components will work best for your unique ELISA needs.ĮLISA builder tool Search ELISA kits Explore ELISA protocols Explore ELISA reagents The below article will guide you through decisions and options for building an ELISA. The most crucial element of an ELISA is a highly specific antibody-antigen interaction. Detection is accomplished by measuring the activity of the reporter enzyme via incubation with the appropriate substrate to produce a measurable product. In an ELISA, the antigen (target macromolecule) is immobilized on a solid surface (microplate) and then complexed with an antibody that is linked to a reporter enzyme. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |